[英文釋義] A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome,encoding the same mutant phenotype,is introduced into that cell.
By using the complementation test an unknown mutant can be rapidly assigned to a particular gene . 采用互補(bǔ)測(cè)驗(yàn)可迅速地將某一未知突變型歸屬于某一特定基因。
Human elongator complex serves a role in chromatin remodeling through modifying the lysine residues of histone h3 and h4 during transcription elongation in higher cells . the main results and conclusions are as followings . 1 using depletion strain of yeast as material, we have established a system for complementation test and gene expression analysis of human elongator complex 4、利用點(diǎn)突變和plasmidshuffle等技術(shù)構(gòu)建了含h3k14一r、h4ks~r單突變和h3k14一側(cè)h4ks一r雙突變的e1p3乙菌株,通過功能互補(bǔ)實(shí)驗(yàn)分析少凡p3對(duì)突變株的補(bǔ)償功能,證實(shí)酵母elongator可對(duì)h314k/h4sk進(jìn)行乙酞化修飾。